Given the right conditions, Adult Stem Cells (ASCs) found in adipose tissue, have the ability to differentiate into cell types such as cartilage, bone and muscle. Endothelium, pancreatic cells, bone, myocardium (heart muscle), liver and neural cells can also be induced given the right conditions in an animal’s body. Changes can be seen in vivo when ASCs are transplanted into tissue environment different than their own origin. Adipose tissue is harvested from the thorax or falciform area of dogs and cats or by a liposuction technique in equines. General anesthesia is required for small animals but a
sedative is all that is required for horses. The tissue is then broken down using patented Medistem™ enzymes, filtered for sterility and washed with a mild antibiotic to give the Stromal Vascular Fraction (SVF), or Medivet Regenerative Cell Fraction (MRCF). Compared to other sources, ASCs found in the collected adipose tissue can range in the millions per unit of measure. The breakdown of cells found in the Stromal Vascular fraction is as follows:
Analysis of the SVF and the derived AT MSC population by using a cytological, flow
cytometrical (FACS) and in vitro assay
Haematopoietic CD45 cells (9%)'
Monocyte progenitor CD14 cells (11%)
Early committed progenitor and mature granulocyte /monocyte CD13 cells (6%)
Haematopoietic CD90 stem cells (29% ) (marker also expressed on connective tissue)
Early lymphohaematopoietic stem and progenitor cells CD34/CD133 (7%).
Endothelial cell-associated markers, including CD31, CD105 and CD146
Endothelial CD45/CD146/UEA-1 cells (8%)
CD271 Cells (0.6%)(Include bone marrow stromal cells, follicular dendritic cells, and mesenchymal cells involved in mesenchymal-epithelial interactions)
Yoshimura, K., Shiguera, T., Matsumoto, D., Sato, T., Takaki, Y., Aiba-Kojima, E., Sato, K., Inoue, K., Nagase, T., Koshima, I. and Gonda, K. 2006 “Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates” Journal of cellular physiology, Vol. 208, pp. 64-76
Coupled with the ease of collection this makes harvesting 1st generation stem cells a superior alternative than previous stem cell therapies that relied on bone marrow collection. Historically, bone marrow derived stem cells can take days or even weeks to culture to ‘therapeutic levels.’ Autologous transplant of adipose-derived stem cells poses an extremely low risk to the patient when completed under strict aseptic conditions. At this time MediVet believes autologous transplant to be the safest and most effective treatment for osteoarthritis, tendon injury, ligament injury and fractures.
LED Light Activation of the MRCF
Low-level light photoactivation can be utilized for significant benefit in the stimulation for enhanced proliferation, differentiation and induction of growth factors and cytokines from cells of any organism. When adipose-derived stem cells are removed from fat tissue using MediVet’s patented enzyme most of the cells are in a dormant, non-dividing state. To be activated within the body there is a repair mechanism cascade that must be activated in order for the stem cells to circulate through the body and begin differentiation into now tissues. Activation for 20 minutes with MediVet’s patented 3 color light stimulates the cells to initiate repair from the moment the cells are injected into the animal’s body.
About MediVet Labs Stem Cell Processing
MediVet Labs is proud to offer Adipose Processing to Veterinarians nationwide. Processing Adipose samples through the Lab is ideal for Veterinarians who would either like to establish their own Case Success before investing in MediVet Equipment and Kits or who do not have the space or other resources to process samples In-Clinic.
Our superior AdiShip Media has been designed to maximize cell viability to the cooled sample. The media is best when refrigerated upon arrival at the Veterinarian’s office. The adipose tissue is placed aseptically in the cold (4*C) media and shipped immediately to MediVet Labs.
The sample is also shipped with payment information and an Adipose Service Form. On this form the Veterinarian indicates how they would like the sample dosed for joint or soft tissue injection. There is also room for the Veterinarian to indicate whether or not extra cells are to be banked or are to be sent back for intravenous use. As always, MediVet Labs will be in direct contact with the Veterinarian if there are any questions about an Adipose sample.
References: Filip S, Mokry J, Hrusks I. Adult Stem Cells for Regenerative Medicine. Circ Res. 2006. 100: 1249-1260.
Yanez R, Lamana ML, Garica-Castro J, et al. Adipose tissue-derived Stem Cells have in vivi immunosppressive properties applicable for the control of graft-versus-host disease. Stem Cells. 2006.
24: 2582-2591
Mvula B, Mathope T, Moore T, Abrahamse H. The effect of low level laser Irradiation on human adipose-derived stem cells. Laser Med Sci. 2008.
