Every item in the procedure is supplied in the Medivet APR kit, except for the centrifuge and sterile forceps. Any standard centrifuge can be used.
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This has been found to be a very practical and cost-effective method for using autologous platelet preparation in a clinical setting.
Requires a simple blood centrifuge.
METHOD (is patent pending by Actistem Ltd)
1.
50 ml venous blood sample is drawn under strict aseptic conditions.
2.
The blood is then injected evenly into 6 ACD Venous Blood Collection Tubes to avoid coagulation.
3.
The tubes are then centrifuged for 15 minutes at 72xg resulting in the three following layers: the inferior layer composed of red cells, the intermediate layer composed of white cells and the superior layer made up of plasma.
4.
The (5ml) plasma layer is removed from each tube with a 50cc sterile syringe attached to a 21 gauge steel catheter.
5.
The plasma is then injected evenly into 4 Plasma Preparation Tubes.
6.
The tubes are then centrifuged for another 5 minutes at 1000xg in order to obtain a two-part plasma: the upper part consisting of 5.5 ml of poor-platelet plasma (PPP) and the lower part consisting of 1.5 ml of platelet-rich plasma (PRP). The plateless poor plasma (PPP) is not retained for use in enhancing the topical wound healing.
7.
The PRP is then gently aspirated with a 10ml syringe attached to a 21 gauge steel catheter and placed evenly in two sterile freezing vial tubes.
8.
The PRP is then prepared for activation by transferring calcium activator (0.5ml) solution with a transfer pipette to each vial. This inhibits the blood-thinning effect of ACD anticoagulant in the venous blood collection tubes.
9.
After activation, PRP turns into a gel-like solution within 25 minutes with adhesive properties and ready for use or for freeze storage.
Medivet is the exclusive global distributor of the MedivetPlatelet Rich Plasma Veterinary Surgical Kit designed as a highly practical and cost effective procedure that can be performed in under an hour to enable veterinarians to produce in house, a Platelet Rich Plasma solution from blood extracted from an animal to be available to be applied topically to a wound site on that animal to control bleeding, seal wounds and accelerate soft tissue healing.
The MedivetPlatelet Rich PlasmaKit eliminates time consuming visits to the blood bank for pre donations. The remarkable benefits of enhanced and superior wound healing simply achieve a markedly improved surgical outcome.
The technology of centrifugal separation makes this incredible service possible.
The MedivetPlatelet Rich PlasmaKit produces Platelet Rich Plasma that is the only available product that contains elevated levels of all the animal’s natural occurring growth factors and in precisely the same ratio as they are found in the body. The Platelet Rich Plasma produced is 100% biocompatible and safe and poses no infection risk as it is generated from the animal's own plasma and can be the single solution to minimise infection, swelling, pain, bruising and scarring whilst hastening wound closure.
